Boswelia Crude Using Vodka

To 550 kg of Boswellia gum resin charged in a suitable extractor was added 1600 litres of ethanol:water (DM) at a ratio 85: 35 by volume. The ethanol was heated to reflux temperature using jacketed steam followed by extracting the gum at reflux condition for 3 hours and cooling the extract to room temperature. The extract was filtered through a micron filter to remove the insoluble particles followed by repeating the extraction twice using 1600 litres of ethanol: water (85: 15). All three filtered extracts was combined and the pH was adjusted to 8.4-8.6 using 25% w/v sodium hydroxide solution. The combined extract was then concentrated in a suitable reactor to approximately 1600 litres by using jacket steam at 60°-85°C under vacuum.

800 litres of water (DM) was added to the concentrated extract to precipitate out the lower terpenes and other gums. This was followed by cooling the mixture to 5-8°C using jacketed chilled water and was maintained at the same temperature for 24 hours. The clear solvent layer was separated from sediments by siphoning and filtering. The solvent layer was concentrated to approximately 1600 litres in a suitable reactor using jacket steam at 60°- 85°C under vacuum. This was followed by addition of 400 litres of water (DM) to the concentrated extract to precipitate the terpenes and other gums. The mixture was cooled to 5-8°C using jacketed chilled water and maintained at the same temperature for another 24 hours.

The clear solvent layer was separated from sediments by_ siphoning and subsequent filtration. The solvent layer was then evaporated in a suitable reactor using jacket steam at 60°-85°C under vacuum to remove the ethanol. The concentrated aqueous layer was diluted with water to get a volume of 2000 litres, followed by acidification with 10% hydrochloric acid to reach a pH upto 2.0. The precipitated Boswellic acids were filtered using a suitable filter which was followed by washing with water (DM) until pH was 6.0- 6.5. The precipitate was dried in an oven at a temperature below 75°C under vacuum to obtain the standardized Boswellic acid fractions.

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